Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: List of experimental groups and concentrations evaluated.

Article Snippet: For experimental groups, KGN (Cat# HY-16268, MedChemExpress, Monmouth Junction, NJ), SM04690 (Cat# HY-109049, MedChemExpress, Monmouth Junction, NJ), CM10 (Sequence LIANAK, Genscript, New Jersey), and/or CK2.1 (Sequence: QIKIWFQNRRK-WKKMVPSDPSYEDMGGC, Genscript, New Jersey) were added to the control medium at concentrations that were based on the literature and summarized in .

Techniques:

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Chondrogenic gene expression of ACAN in hBMSCs spheroids after 21 days in culture. No statistically significant differences were observed between groups except for TGF-β3, which had significantly higher aggrecan expression compared to all other groups, including a 10,000-fold larger value compared to the negative control. ∗∗∗∗Refers to significant difference p ​< ​0.0001, n ​= ​6–8. Reported values are mean ​+ ​standard deviation. SM ​= ​SM04690, CK ​= ​CK2.1, CM ​= ​CM10, CM/CK (100/100) ​= ​CM10 at 100 ​μM and CK2.1 and 100 ​nM, CM/CK(100/500) ​= ​CM10 at 100 ​μM and CK2.1 and 500 ​nM, CM/CK (200/100) ​= ​CM10 at 200 ​μM and CK2.1 and 100 ​nM, CM/CK(200/500) ​= ​CM10 at 200 ​μM and CK2.1 and 500 ​nM, CM/KGN (100/1) ​= ​CM10 at 100 ​μM and kartogenin at 1 ​μM, CM/KGN (100/10) ​= ​CM10 at 100 ​μM and kartogenin at 10 ​μM, CK/KGN (100/1) ​= ​CK2.1 ​at 100 ​nM and kartogenin at 1 ​ ​μM, CK/KGN (100/10) ​= ​CK2.1 ​at 100 ​nM and kartogenin at 10 ​μM, CM/SM (100/30) ​= ​CM10 at 100 ​μM and SM04690 at 30 ​nM, CM/SM (100/100) ​= ​CM10 at 100 ​μM and SM04690 at 100 ​nM, CK/SM (100/30) ​= ​CK2.1 at 100 ​nM and SM04690 at 30 ​nM, CK/SM (100/100) ​= ​CK2.1 at 100 ​nM and SM04690 at 100 ​nM.

Article Snippet: For experimental groups, KGN (Cat# HY-16268, MedChemExpress, Monmouth Junction, NJ), SM04690 (Cat# HY-109049, MedChemExpress, Monmouth Junction, NJ), CM10 (Sequence LIANAK, Genscript, New Jersey), and/or CK2.1 (Sequence: QIKIWFQNRRK-WKKMVPSDPSYEDMGGC, Genscript, New Jersey) were added to the control medium at concentrations that were based on the literature and summarized in .

Techniques: Gene Expression, Expressing, Negative Control

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Chondrogenic gene expression of SOX-9 in hBMSCs spheroids after 21 days in culture. No significance was observed between groups except for TGF-β3 with a 50-fold higher gene expression as compared to the negative control. ∗∗∗∗Refers to significant difference p ​< ​0.0001, n ​= ​6–8. Reported values are mean ​+ ​standard deviation. SM ​= ​SM04690, CK ​= ​CK2.1, CM ​= ​CM10, CM/CK (100/100) ​= ​CM10 at 100 ​μM and CK2.1 and 100 ​nM, CM/CK(100/500) ​= ​CM10 at 100 ​μM and CK2.1 and 500 ​nM, CM/CK (200/100) ​= ​CM10 at 200 ​μM and CK2.1 and 100 ​nM, CM/CK(200/500) ​= ​CM10 at 200 ​μM and CK2.1 and 500 ​nM, CM/KGN (100/1) ​= ​CM10 at 100 ​μM and kartogenin at 1 ​μM, CM/KGN (100/10) ​= ​CM10 at 100 ​μM and kartogenin at 10 ​μM, CK/KGN (100/1) ​= ​CK2.1 at 100 ​nM and kartogenin at 1 ​ ​μM, CK/KGN (100/10) ​= ​CK2.1 at 100 ​nM and kartogenin at 10 ​μM, CM/SM (100/30) ​= ​CM10 at 100 ​μM and SM04690 at 30 ​nM, CM/SM (100/100) ​= ​CM10 at 100 ​μM and SM04690 at 100 ​nM, CK/SM (100/30) ​= ​CK2.1 at 100 ​nM and SM04690 at 30 ​nM, CK/SM (100/100) ​= ​CK2.1 at 100 ​nM and SM04690 at 100 ​nM.

Article Snippet: For experimental groups, KGN (Cat# HY-16268, MedChemExpress, Monmouth Junction, NJ), SM04690 (Cat# HY-109049, MedChemExpress, Monmouth Junction, NJ), CM10 (Sequence LIANAK, Genscript, New Jersey), and/or CK2.1 (Sequence: QIKIWFQNRRK-WKKMVPSDPSYEDMGGC, Genscript, New Jersey) were added to the control medium at concentrations that were based on the literature and summarized in .

Techniques: Gene Expression, Negative Control

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Gene expression of collagen I of hBMSCs spheroids after 21 days in culture. A variation in the gene expression of collagen I by hBMSCs was observed in response to the evaluated compounds and peptides. CM10 (100 ​μM) had the highest gene expression with 2.5-fold higher expression compared to the control group. CK2.1 exhibited higher gene expression of collagen I by 1.8 folds and 1.4 folds at 100 ​nM and 500 ​nM, respectively. The combination of compounds and peptides did not induce a significant difference in collagen I gene expression as compared to the negative control group. Groups with the same letters indicate no significance, n ​= ​6–8. Reported values are mean ​+ ​standard deviation. SM ​= ​SM04690, CK ​= ​CK2.1, CM ​= ​CM10, CM/CK (100/100) ​= ​CM10 at 100 ​μM and CK2.1 and 100 ​nM, CM/CK(100/500) ​= ​CM10 at 100 ​μM and CK2.1 and 500 ​nM, CM/CK (200/100) ​= ​CM10 at 200 ​μM and CK2.1 and 100 ​nM, CM/CK(200/500) ​= ​CM10 at 200 ​μM and CK2.1 and 500 ​nM, CM/KGN (100/1) ​= ​CM10 at 100 ​μM and kartogenin at 1 ​μM, CM/KGN (100/10) ​= ​CM10 at 100 ​μM and kartogenin at 10 ​μM, CK/KGN (100/1) ​= ​CK2.1 at 100 ​nM and kartogenin at 1 ​ ​μM, CK/KGN (100/10) ​= ​CK2.1 at 100 ​nM and kartogenin at 10 ​μM, CM/SM (100/30) ​= ​CM10 at 100 ​μM and SM04690 at 30 ​nM, CM/SM (100/100) ​= ​CM10 at 100 ​μM and SM04690 at 100 ​nM, CK/SM (100/30) ​= ​CK2.1 at 100 ​nM and SM04690 at 30 ​nM, CK/SM (100/100) ​= ​CK2.1 at 100 ​nM and SM04690 at 100 ​nM.

Article Snippet: For experimental groups, KGN (Cat# HY-16268, MedChemExpress, Monmouth Junction, NJ), SM04690 (Cat# HY-109049, MedChemExpress, Monmouth Junction, NJ), CM10 (Sequence LIANAK, Genscript, New Jersey), and/or CK2.1 (Sequence: QIKIWFQNRRK-WKKMVPSDPSYEDMGGC, Genscript, New Jersey) were added to the control medium at concentrations that were based on the literature and summarized in .

Techniques: Gene Expression, Expressing, Control, Negative Control

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Gene expression of ACAN, SOX-9, and collagen I of hBMSCs spheroids from 3 different donors after 21 days in culture. For ACAN, no statistically significant differences were observed between groups except for TGF-β3 with all donors. Donor-based variation in the gene expression of SOX-9 in response to experimental groups was observed. Notably, CM10 had a significantly higher gene expression of SOX-9 as compared to the negative control only with donor 1 (Female, 20 ​yrs). SM04690, CM10, and CK2.1 induced a significantly lower gene expression of collagen I as compared to the negative control with all donors, whereas KGN had variable outcomes based on the donor. Groups with the same letters indicate no significance, n ​= ​6–8. Reported values are mean ​+ ​standard deviation. SM ​= ​SM04690, CK ​= ​CK2.1, CM ​= ​CM10.

Article Snippet: For experimental groups, KGN (Cat# HY-16268, MedChemExpress, Monmouth Junction, NJ), SM04690 (Cat# HY-109049, MedChemExpress, Monmouth Junction, NJ), CM10 (Sequence LIANAK, Genscript, New Jersey), and/or CK2.1 (Sequence: QIKIWFQNRRK-WKKMVPSDPSYEDMGGC, Genscript, New Jersey) were added to the control medium at concentrations that were based on the literature and summarized in .

Techniques: Gene Expression, Negative Control